Goals To determine expression and localization of Wnt signaling molecules across
Goals To determine expression and localization of Wnt signaling molecules across the esophageal mucosal thickness. protein 1, T-cellCspecific transcription Compound W manufacture factor 3, and dishevelled 3 were expressed highest in LP decreasing precipitously medially toward SC. Dkk1 predominantly expressed in SC was more than 100-folds greater than other layers (statistics or Mann-Whitney Rank Sum test for nonparametric data was used subsequently to determine differences between the layers. RESULTS Real-time PCR Analysis Real-time PCR analysis of the LCM-generated (Fig. 1) samples, demonstrated differential expression of the Wnt signaling components throughout the thickness of the squamous mucosa. Different magnitudes of expression of Wnt ligands (Wnt 1, 2b, 3, 3a, 5a, 5b), receptors [FZD 1, low-density lipoprotein receptor-related protein 6 (LRP 6)], modulating proteins (Dkk 1, 3, 4, SFRP 1), and intracellular components [TCF 3, dishevelled (DVL) 3] were detected in all layers. Wnt Ligand Expression Wnt 1 Wnt 1 expression was significantly different between the different layers (embryos. Compound W manufacture Cell. 1996;86:391C399. [PubMed] 31. Golan T, Yaniv A, Bafico A, et al. The Human Frizzled 6 (HFz6) acts as a negative regulator of the canonical Wnt–catenin signaling cascade. JBC. 2004;279:14879C14888. [PubMed] 32. Lyons JP, Mueller UW, Ji H, et al. Wnt-4 activates the canonical -catenin-mediated Wnt pathway and binds Frizzled-6 CRD: function implications of Wnt/-catenin activity in kidney epithelial cells. Exp Cell Res. 2004;298:369C387. [PubMed] 33. Cui C-Y, Hashimoto T, Grivennikov SI, et al. Ectodysplasin regulates the lymphotoxin- pathway for hair differentiation. Compound W manufacture PNAS. 2006;103:9142C9147. [PMC free article] [PubMed] 34. Van der Horst G, van der Werf S, Farih-Sips H, et al. Downregulation of Wnt signaling by increased expression of Dickkopf-1 and -2 is a prerequisite for late-stage osteoblast differentiation of KS483 cells. J Bone Min Res. 2005;20:1867C1877. [PubMed] 35. Yamaguchi Y, Passeron T, Watabe H, et al. The effect of Dickkopf KIAA1819 1 on gene expression and Wnt signaling by melanocytes: mechanisms underlying its suppression of melanocyte function and proliferation. J Inves Derm. 2007;127:1217C1225. [PubMed] 36. Shou J, Ali-Osman F, Multani AS, et al. Human Dkk-1, a gene encoding a Wnt antagonist, responds to DNA damage and its overexpression sensitizes brain tumor cells to apoptosis following alkylation damage of DNA. Oncogene. 2002;21:878C889. [PubMed] 37. Wang J, Shou J, Chen X. Dickkopf 1 an inhibitor of the Wnt pathway, is induced by p53. Oncogene. 2000;19:843C1848. Compound W manufacture [PubMed] 38. Wetscher GJ, Schwelberger H, Unger A, et al. Reflux-induced apoptosis of the esophageal mucosa is inhibited in Barrett’s epithelium. Am J Surg. 1998;76:569C573. [PubMed] 39. Roman-Gomez J, Jimenez-Velasco A, Agirre X, et al. Transcriptional silencing of the Dickkopf-3 (Dkk-3) gene by CpG hypermethylation in acute lymphoblastic leukaemia. Br J Cancer. 2004;91:707C713. [PMC free article] [PubMed] 40. Hsieh SY, Hsieh PS, Chiu CT, et al. Dickkopf-3/REIC functions as a suppressor gene of tumor growth. Oncogene. 2004;23:9183C9189. [PubMed] 41. Melkonyan HS, Chang WC, Shapiro JP, et al. SARPs: a family of secreted apoptosis-related proteins. Proc Natl Acad Sci. 1997;94:13636C13641. [PMC free article] [PubMed] 42. Veeck J, Niederacher D, An H, et al. Aberrent methylation of the Wnt antagonist SFRP1 in breast cancer is associated with unfavourable prognosis. Oncogene. 2006;25:3479C3488. [PubMed].